Rx::GFP genetically modified stem cells (RIKKEN Kobe institute)

Cells are maintained on gelatin-coated dishes in GMEM supplemented with FBS (1%), KSR (10% ), glutamine (2 mM), nonessential amino acids (0.1 mM ), pyruvate (1 mM), 2-mercaptoethanol (2-ME. 0.1 mM), and LIF (2000 U/ml).