Human bronchial smooth muscle cells were obtained from surgical waste lung tissue collected from patients undergoing thoracic surgery. The airway smooth muscle cell phenotype was identified by immunostaining for smooth muscle actin and myosin and by expression of receptors (M3 muscarinic, bradykinin, and histamine) for agonists commonly used for the airway.
Human bronchial smooth muscle cells were obtained from surgical waste lung tissue from patients undergoing thoracic surgery. Tissues were initially placed in Hank's balanced salt solution (HBSS) with extracellular Ca2+ (2.5 mM). Bronchioles were identified and freed of cartilage, epithelium, and surrounding tissues, and airway smooth muscle (ASM) cells were isolated. Cells were plated in DMEM-Ham's F-12 medium supplemented with FBS (10%).