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Wharton-Jelly-derived mesenchymal stem cells (Qingdao University)

Wharton-jelly mesenchymal stem cells were obtained from the umbilical cord of a healthy donor.

The umbilical cord is washed twice with phosphate buffered saline (PBS) and then dissected with scissors into pieces (≈1cm3). These are plated in a cell culture dish containing low glucose Dulbecco`s Modified Eagle`s Medium medium supplemented with non-animal-derived serum (5%). Cells are maintained in a humidified atmosphere with 5% CO2 at 37°C. After 3 days of culture, the medium is replaced to remove the tissue and non-adherent cells, and changed twice weekly thereafter. Once 80% confluence is reached, the adherent cells (passage 0) are detached with trypsin (0.125%) and passaged in the cell culture dish.

See additional Stem, Progenitor & Primary Cells for: Mesenchymal Stem Cells , Umbilical Cord
Adult Stem / Progenitor Cell
Homo sapiens
Wharton-Jelly-derived mesenchymal stem cells