Wharton's jelly-derived mesenchymal stem cells (General Hospital of Chinese PLA)

Umbilical cords were rinsed several times in sterile phosphate-buffered saline (PBS) to remove blood components, and then cut into small pieces (2–3 cm). Cord vessels were removed to avoid endothelial cell contamination and the Wharton's jelly parts of the cord were cut into small pieces and seeded  into tissue-culture flasks in low-glucose Dulbecco's modified Eagle's medium (LG-DMEM) supplemented with  fetal bovine serum (10%, FBS), penicillin/streptomycin (100 U/ml ).