Wharton's jelly-derived mesenchymal stem cells (Institute of Cell Biology and Neurobiology)

Wharton's jelly mesenchymal stem cells (WJ-MSCs) were derived from Wharton's jelly of 39-40 week (gestational age) human umbilical cords (UCs), within 2-4 hours from collection time. UCs were washed, rinsed in ethanol (70%) for 30 seconds, cut, treated with collagenase for 16 hours and digested in trypsin for 30 minutes. Isolated cells were plated and cultured in Dulbecco’s Modified Eagle Medium Low Glucose (DMEM-LG) supplemented with fetal bovine serum (FBS, 10%), and penicillin/streptomycin (100 U/ml). Medium was exchanged every 3 days.