Wharton's jelly-derived mesenchymal stem cells (National Yang Ming University)

Wharton's jelly mesenchymal stem cells (WJ-MSCs) were derived from Wharton's jelly (WJ) of human umbilical cords (UCs) processed within 24 hours of collection time. WJ cells were digested with collagenase and trypsin, washed twice in phosphate-buffered saline (PBS), plated and cultured in MesenCult^TM medium.  Nonadherent cells were removed the next day by medium exchange. The remaining adherent fibroblast-like cells were passaged after 4-7 days.