Skeletal myoblasts (MyoCell®) (Bioheart, Inc)

The biopsies are finely minced and then dissociated using digestive enzymes. The dissociated tissue is washed several times and filtered until a single-cell suspension is achieved. The cells are plated, in cell culture dishes, in SkM basal growth medium supplemented with fetal bovine serum (15-20%), recombinant human EGF (10 μg/ml) and dexamethasone (2 μg/ml). The medium is changed at regular intervals; the cells are harvested and replated according to cell confluence parameters. Final harvest is done after three to five passages.