All in Vitro Cells > Adult Stem / Progenitor Cell card

Dental pulp stem cells (Dayananda Sagar College of Dental Sciences)

Dental pulp stem cells were isolated from permanent teeth. Cells express  markers, and can into osteoblasts and adipocytes.

Teeth surfaces were cleaned and cut to reveal the pulp chamber, and pulp tissue was then gently isolated and placed in PBS solution. The tissue was digested in a solution of collagenase (3 mg/ml) and dispase (4 mg/ml) for 1 h. Cells were removed and resuspended in Dulbecco’s PBS (DPBS) and centrifuged at 1,800 rpm for 5 min to pellet the cells. Cells were collected and resuspended in DMEM knockout medium and cultured for several days to promote cell adhesion. The medium was then replaced with complete DMEM knockout medium supplemented with FBS (10%)

See additional Stem, Progenitor & Primary Cells for: Tooth
Adult Stem / Progenitor Cell
Homo sapiens
Dental pulp stem cells