Dental pulp stem cells were isolated from healthy teeth. Cells express mesenchymal markers and can differentiate into cartilage tissue in-vitro.
Dental pulp tissue was isolated from premolar teeth scheduled to be removed for orthodontic purposes. The teeth were washed in phosphate buffered saline containing penicillin and streptomycin, and pulps were exposed using a cylindrical diamond bur mounted on a high-speed hand piece. Pulps were removed, cut into small pieces and then cultured in mesenchymal stem cell medium (MSCM).