Dental pulp stem cells (Manipal Institute of Regenerative Medicine)

Isolated pulp tissues were minced and digested in a collagenase mixture for 1 hour, and centrifuged to obtain tissue pellets. Cells were cultured in knockout Dulbecco’s modified Eagle’s medium (KO-DMEM), supplemented with fetal bovine serum (10%), L-glutamine (5 mM) and penicillin-streptomycin (50 units/ml).