EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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Stem cells from human exfoliated deciduous teeth (Capital Medical University School of Stomatology)

Stem cells from exfoliated deciduous teeth (SHED) are isolated from upper and lower incisors of 7- to 8-year-old children. 


Pulp tissue of normal exfoliated human deciduous incisors was digested in a solution of  collagenase type I (3 mg/ml) and  dispase (4 mg/ml) for 1 hour. Single-cell suspensions were seeded in tissue culture plates and cultured in minimal essential medium, α-modification (α-MEM) supplemented with  FCS (20%),  l-ascorbic acid 2-phosphate (100 μM),  L-glutamine (2 mM), penicillin (100 units/ml), and streptomycin (100 μg/ml).

See additional Stem, Progenitor & Primary Cells for: Tooth
Adult Stem / Progenitor Cell
Homo sapiens
Capital Medical University School of Stomatology
Stem cells from human exfoliated deciduous teeth