Stem cells from exfoliated deciduous teeth (SHED) are isolated from upper and lower incisors of 7- to 8-year-old children.
Pulp tissue of normal exfoliated human deciduous incisors was digested in a solution of collagenase type I (3 mg/ml) and dispase (4 mg/ml) for 1 hour. Single-cell suspensions were seeded in tissue culture plates and cultured in minimal essential medium, α-modification (α-MEM) supplemented with FCS (20%), l-ascorbic acid 2-phosphate (100 μM), L-glutamine (2 mM), penicillin (100 units/ml), and streptomycin (100 μg/ml).