Directed differentiation of functional astroglial subtypes from human pluripotent stem cells
This protocol describes the direct differentiation of astrocytes from human pluripotent stem cells (hPSC), using a chemically defined system. These astrocytes-like cells exhibit specific regional and functional characteristics and functional properties manifested by neurotransmitter and ion uptake, support of neuronal maturation and engraftment into mouse brain.
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Directed differentiation of functional astroglial subtypes from human pluripotent stem cells