Renal multipotent progenitor cell (CD24+CD133+) isolation from human embryonic kidneys

This protocol describes isolation of CD24
+CD133+ cells from human embryonic kidneys. CD24+
CD133+
cells constitute the early primordial nephron, but progressively disappear during nephron development until they become selectively localized at the urinary pole of Bowman’s capsule. When isolated and injected into SCID mice with acute renal failure from glycerol-induced rhabdomyolysis, these cells regenerate different portions of the nephron, reduce tissue necrosis and fibrosis, and significantly improve renal function.