IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
This induced pluripotent stem cell (iPSC) line was generated from embryonic fibroblasts by transduction of four factors: OCT3/4, SOX2, KLF4 and c-MYC. The cells express GFP under the NANOG promoter (Nanog-GFP-IRES-Puro resistance).
Cells are cultured on feeder cells (irradiated mouse embryonic fibroblasts (MEFs)) in DMEM (high glucose, without sodium pyruvate) supplemented with FBS (15%), NEAA (0.1mM), 2-mercaptoethanol (0.1mM) and mouse LIF (1000U/ml).