The AS034 cell line was derived from pronase-treated blastocysts and demonstrates alkaline phosphtase expression, detected via staining. The line was tested in vitro for formation of all three germ layers; it forms teratomas. Karyotype: 46,XY
Routinely passage every 4–5 days. hES cell colonies can be mechanically cut into pieces, 200 × 200 μm, removed from the culture dish and transferred to a new culture dish with fresh MEF cells and VitroHES supplemented with 4 ng/ml human recombinant bFGF (hrbFGF).