This induced pluripotent stem cell (iPSC) line was generated from adult human fibroblasts transduced with retrovial vectors containing the human OCT4, SOX2, KLF4, and MYC genes. Cells exhibit a normal karyotype, 46,XX.
Transduced fibroblasts were cultured in DMEM supplemented with Medium 199 (20%) containing Earl's salts, FCS (10%), Glutamax (2 mM), and β-mercaptoethanol (50 μM). After 5 days, iPS colonies were picked and transferred to matrigel-coated plates and cultured in mTeSR medium.