Wharton's jelly-derived mesenchymal stem cells (Isfahan University of Medical Sciences)

Isolated Wharton's jelly (WJ) was cut into small pieces and plated in culture medium comprised of Dulbecco’s modified Eagle’s medium low-glucose (DMEM-LG) supplemented with fetal bovine serum (FBS, 10%), L-glutamine (2 mM), penicillin (100 U/ml), streptomycin (100 μg/ml), Fungizone (25 μg/ml), basic fibroblast growth factor (bFGF, 5 ng/ml) and epidermal growth factor (EGF, 5 ng/ml). The first medium change was after 4 days, followed by medium changes every 3-4 days until cells were confluent.  Cells were passaged, using trypsin with diamine tetraacetic acid (EDTA), and maintained in culture medium.