IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
MyoCell® are skeletal myoblasts obtained from the quadriceps muscle and then cultured in vitro for cell expansion.
The biopsies are finely minced and then dissociated using digestive enzymes. The dissociated tissue is washed several times and filtered until a single-cell suspension is achieved. The cells are plated, in cell culture dishes, in SkM basal growth medium supplemented with fetal bovine serum (15-20%), recombinant human EGF (10 μg/ml) and dexamethasone (2 μg/ml). The medium is changed at regular intervals; the cells are harvested and replated according to cell confluence parameters. Final harvest is done after three to five passages.