Purification of stem cell-derived dopaminergic neurons by isolation of NCAM1-positive cells
The following protocol describes the generation and isolation of ventral midbrain dopaminergic neurons from stem cells, by administration of defined growth factors and isolation of NCAM1+/CD29 cells. The isolated cells improve behavioral outcomes of the parkinsonian rat and integrate into the brain of the cynomolgus macaque.
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Purification of stem cell-derived dopaminergic neurons by isolation of NCAM1-positive cells