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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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Welcome to
LifeMap Discovery

Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine

Generation of intermediate mesoderm cells from human pluripotent stem cells

This protocol describes a method to generate intermediate mesoderm (IM) cells, which can be sorted and induced to further differentiate into various cells of IM-derived lineages. This highly efficient protocol (more than 90% of the IM cells express OSR1) is based on 2D-differentiation of single embryonic stem cell cultures in serum-free medium. 
11-18 days
See additional Protocols for: Kidney , Mesoderm
Generation of intermediate mesoderm cells from human pluripotent stem cells