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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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Derivation of neural crest cells and their progeny (MS-5 feeder cells/feeder free) from hESC

This is a description of two distinct methods to differentiate hESC into neural crest cells. One protocol uses MS-5 cells as a feeder layer and the second is a feeder-free protocol.  Additional differentiation steps toward neural crest progeny are also detailed.

14-35 (14-81)  days
See additional Protocols for: Neural Crest , Neural Ectoderm
Derivation of neural crest cells and their progeny  (MS-5 feeder cells/feeder free) from hESC