Myoblasts are mononucleated undifferentiated muscle cells that express myogenic regulatory factors (MRFs). The formation of myoblasts marks the initiation of the early stages of myotomal differentiation. Myoblasts constantly proliferate to provide sufficient number of cells for myogenic differentiation and for continuous muscle growth. Differentiation strarts in the proximal mesenchyme and progresses distally as the limb bud develops. Therefore, myogenically committed cells are found towards the center of the limb bud.