Adipose-derived stem cells (ADSCs) are isolated from fat tissue and are suitable for autologous transplantation toward the treatment of premature ovarian failure. These cells are identified by the presence of the markers CD29 and CD44.
ADSCs were isolated from 100ml fat harvested from the abdomen of premature ovarian failure patients, using an abdominal fat abortion technique. ADSCs were isolated and cultured in-vitro in autologous serum. The biomarkers CD29 and CD44, were detected using flow cytometry.