Adipose-derived mesenchymal stromal cells (University Hospital, Montpellier)

Fat was digested with collagenase, at 37°C for 45 minutes and centrifuged at 600g for 10 minutes. The stromal vascular fraction was transferred to a CellStack chamber, at a density of 4,000 cells/cm2, in minimum essential medium (MEM) supplemented with platelet lysate. On day 8, the cells were trypsinized and expanded at a density of 2,000 cells/cm2 until day 14.