Bone marrow-derived mesenchymal stem cell line MSCB9 (University of Texas Southwestern Medical Center)

The bone marrow of the femor and tibia bones of mice was flushed out and the cells were cultured in mesenchymal stem cell expansion medium comprised of low-glucose Dulbecco's modified Eagle's medium supplemented with Molecular Cell Developmental Biology (MCDB)-201 medium (40% ), fetal bovine serum (FBS, 10% ), insulin–transferrin–selenium (ITS; X1),  linoleic-acid–bovine serum-albumin (10%), dexamethasone (10⁻⁸ M), ascorbic acid 2-phosphate (10⁻⁴M),  penicillin/streptomycin (50 U/ml) and the following growth factors: recombinant human platelet-derived growth factor-BB (hPDGF-BB; 10 ng/ml ), recombinant murine epidermal growth factor (mEGF; 10 ng/ml), and recombinant murine leukemia inhibitory factor (LIF, 1000 U/ml).