Primary neuron cultures were obtained from fetal brain tissue collected between 14 and 16 weeks of development. Neuronal cultures of 90% purity were obtained by day 7 of treating mixed primary cultures with fluorodeoxyuridine (FdU). The neurons were well differentiated and established healthy networks of neurites.
Neurons were isolated from the brain tissue of 14-18-week-old human fetuses. The cerebral tissue was minced in serum-free neuronal medium (MEM in Earle’s balanced salt containing sodium bicarbonate (0.225%), sodium pyruvate (1 mM), L-glutamine (2 mM), dextrose (0.1%), 1 X antibiotic Pen-Strep) and dissociated by trypsin treatment. The cells are plated on poly-lysine-coated dishes at a density of 3 X 106 cells/ml in non-complemented medium+serum (5%). 5’ fluoro-2’-deoxyuridine (FdU, 1mM) was added in fresh medium after 4, 7, and 10 days in culture.