All in Vitro Cells > Induced Pluripotent Stem Cell card

201B6 (International Stem Cell Registry)

This induced pluripotent stem cell (iPSC) line was derived from human dermal fibroblasts by lentiviral transduction of  OCT4, SOX2, KLF4, and c-MYC. Karyotype: Normal, 46XX.

Growth conditions: Generate and maintain iPS cells in primate ES medium (ReproCELL, Japan) supplemented with recombinant human basic fibroblast growth factor (bFGF, 4 ng/ml). Seed iPS cells on Matrigel-coated plates in mouse embryonic fibroblast (MEF)-conditioned primate ES cell medium, supplemented with bFGF (4 ng/ml). Change the medium daily. For preparation of MEF-conditioned medium: Plate MEFs derived from embryonic day 13.5 embryo pool of ICR mice at 1*10^6 cells per 100 mm dish and incubate overnight. Then, wash the cells once with PBS and culture in 10 ml primate ES cell medium. After a 24 hr incubation, collect the supernatant of the MEF culture, filter through a 0.22 mm pore-size filter and store.

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Induced Pluripotent Stem Cell
Homo sapiens