CyT49 (ViaCyte - (Novocell) San Diego, CA)

Culture cells in Xeno-free growth media (XF HA): DMEM/F12 containing GlutaMAX supplemented with Xeno-free KnockOut Serum Replacement1 (0% (v/v)), non-essential amino acids (1% (v/v)), 2-mercaptoethanol (0.1 mM), penicillin/streptomycin (1% (v/v)), heregulin-1β (10 ng/mL) and activin A (10 ng/mL).