EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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All in Vitro Cells > Induced Pluripotent Stem Cell card

L1B34 (Osaka University Graduate School of Medicine)

The L1B34 line of induced pluripotent stem cells was derived from corneal limbal epithelial cells by lentiviral transduction of OCT3/4, SOX2, C-MYC, and KLF4. Cells express pluripotency markers and have a normal karyotype, 46,XX.


Cells were seeded on MMC-treated mouse embryonic fibroblast (MEF) feeder cells and cultured in embryonic stem cell culture medium comprised of DMEM/F12  supplemented with knockout serum replacement (20%), β-mercaptoethanol (0.1 mM), non-essential amino acid (0.1 mM), and bFGF (4 ng/ml). 

See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Induced Pluripotent Stem Cell
Homo sapiens
Osaka University Graduate School of Medicine
L1B34
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