This human ES cell line expresses cell surface markers specific to undifferentiated nonhuman primate ES and human EC cells: : stage-specific embryonic antigen (SSEA)–3, SSEA-4, TRA-l-60, TRA-1- 81, and alkaline phosphatase. It has a normal 46, XY karyotype, O+ blood type. Teratomas are formed upon cell injection into SCID mice. These teratomas include cells from all three germ layers.
This cell line can be cultured using feeder cells or using a feeder-free protocol. When using MEF feeder cells, ES medium should contain KNOCKOUT–Dulbecco's modified Eagle's medium (KO-DMEM), supplemented with KNOCKOUT serum replacement (20%), L-glutamine (1 mM), b-mercaptoethanol (0.1 mM), nonessential amino acids (1%), and human basic fibroblast growth factor (hbFGF, 4 ng/ml).
EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
