Reprogrammed alpha1-antitrypsin deficiency cells (University of Cambridge)

Induced pluripotent stem cells (iPSC) were derived from a male with clinical features of α1-antitrypsin deficiency (A1ATD). The donated tissue was infected with a mix of moloney murine leukemia virus-derived vectors, each containing the coding sequences of one of the following human genes: OCT4, SOX2, c-MYC, and KLF4. Cells express pluripotency markers, demonstrate alkaline phosphatase activity, and form teratomas which contain cells from all the three germ layers.