This stem cell line was derived from the inner cell mass cells of cryopreserved pronuclear-stage embryos. Cells express pluripotent markers. Cells have a normal karyotype, 46,XY.
Cells are maintained on STO (mouse fibroblasts) feeder cells in knockout
Dulbecco’s modified Eagle’s medium (KO-DMEM) or
DMEM/F12 (Invitrogen) supplemented with knockout
serum replacement (KSR,20% ), basic fibroblast
growth factor (bFGF,0.4 ng/ml), L-glutamine
(2 mM), nonessential amino acid (1% ),
β-mercaptoethanol (0.1 mM), penicillin (50 U/ml ),
and streptomycin (50 μg/ml).