A two-step small molecules protocol for derivation of endoderm and pancreatic progenitor cells
This protocol describes two small molecules, IDE1 and IDE2, that can efficiently induce definitive endoderm from mouse and human embryonic stem cells (ESCs). In addition, another small molecule, Indolactam V, is used to induce further differentiation into pancreatic progenitor cells.
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A two-step small molecules protocol for derivation of endoderm and pancreatic progenitor cells