eNOS-transfected endothelial progenitor cells (Ottawa Hospital Research Institute)

Collected MNCs will be enriched, by density gradient centrifugation, followed by differential culture, on fibronectin-coated plates in the presence of human recombinant growth factors (vascular endothelial growth factor-A, insulin-like growth factor, epidermal growth factor and fibroblast growth factor-2). After 2-3 days, cells will be transfected with a human eNOS-pVAX plasmid complexed with linear polyethylenimine (jetPEI).