Peripheral blood-derived endothelial progenitor cells (University of Frankfurt)

Mononuclear cells were isolated from peripheral blood, by density-gradient centrifugation with Biocoll. After isolation, mononuclear cells were plated on 24-well human fibronectin- and gelatin-coated culture dishes and cultured in endothelial basal medium (EBM,) supplemented with endothelial cell growth medium (EGM) supplements and  FCS (20%). After 4 days in culture, nonadherent cells were removed by washing with PBS, and adherent cells were analysed.