Genetically modified vein-derived endothelial cells (Multi Gene Vascular Systems (MGVS))

Endothelial cells (EC) were isolated from the  jugular vein or the femoral artery following the surgery for ischemia induction. After cell expansion to at least 5 × 10⁶ EC, cells were transduced. Pseudo-typed retroviral vectors encoding Ang-1-GFP were used for gene transfer to EC. Average time from cell isolation to completion of expansion and gene transfer was 40 days (range 31–55).