The clonal human endothelial progenitor line 05 was derived from the registered parental hES cell line ESI-017. Derivation was performed using a modified protocol for EB differentiation with differentiation medium supplemented, in a step-wise fashion, with BMP4, activin A, FGF2, VEGF-A and the TGFb inhibitor - SB431542. This line expresses vascular endothelium cell surface markers such as, vWF, TEK, PECAM1, CD34, CDH5, KDR, as well as early endothelial markers such as, TAL1. This line is also shows an undifferentiated venous/arterial stage characterized by expression of both arterial markers, such as, EFNB2, NRP1 and venous/lymphatic markers such as, LyVE1, EPHB4, NRP2 and SOX18, as well as the endodermal marker Sox17.
EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
